PNC-27 dosage for a large / giant breed
over 77 lb (35 kg)
The short answer
For a dog weighing over 77 lb (35 kg), the dose of PNC-27 reported in the literature is 20-150 mg per dose, given 2-3x weekly, over 8-12+ weeks. It is studied in this weight class primarily for investigational oncology adjunct, veterinary supervision required. These figures are reported as published and are not recommendations — the dose for an individual dog must be set by a veterinarian who has examined the animal.
Worked examples at specific weights
Calculated from the reported range of 0.5–2 mg per kg of body weight.
| Body weight | Calculated dose range | Frequency |
|---|---|---|
| 40 kg (88 lb) | 20–80 mg | 2-3x weekly |
| 50 kg (110 lb) | 25–100 mg | 2-3x weekly |
| 60 kg (132 lb) | 30–120 mg | 2-3x weekly |
For an exact figure at your dog's weight, use the dose calculator.
Typical breeds in this weight class
German Shepherd, Labrador Retriever, Rottweiler, Great Dane, Mastiff and similarly sized dogs generally fall within over 77 lb (35 kg). Weigh your dog rather than estimating — dosing is calculated per kilogram and an estimate that is out by a few kilos changes the figure materially, particularly at the smaller end.
Before dosing at all
⚠ No companion animal data exists
Every published finding on PNC-27 comes from cell culture or rodent models. There are no canine trials, no canine pharmacokinetics, and no canine safety data. This compound is included for completeness of the research landscape, not as a recommendation. It should only ever be considered under direct veterinary oncology supervision.
Common questions
How much PNC-27 for a large / giant breed?+
For a dog in the over 77 lb (35 kg) range, the reported dose is 20-150 mg per dose, given 2-3x weekly, typically for 8-12+ weeks. At a representative weight of 45 kg (about 99 lb), that works out to roughly 22.5–90 mg per dose. These figures are reported from the source literature and are not recommendations.
How often should a large / giant breed be given PNC-27?+
2-3x weekly is the reported frequency, over a course of 8-12+ weeks. See the cycling guide for how breaks are structured.
Is PNC-27 safe for a large / giant breed?+
There is no honest way to characterise PNC-27 as safe or unsafe for dogs, because no dog has been formally studied. All published evidence is in vitro and rodent. The mechanism is genuinely interesting and the preclinical selectivity data is real, but the gap between that and a canine safety profile is enormous. This belongs in the hands of a veterinary oncologist making a considered decision about a specific patient, not in a general protocol.
References
- 1.Sarafraz-Yazdi E, et al. Anticancer peptide PNC-27 adopts an HDM-2-binding conformation and kills cancer cells by binding to HDM-2 in their membranes. PNAS, 2010. PMID 20080606
- 2.Sookraj KA, et al. The anti-cancer peptide PNC-27 induces tumor cell lysis as the intact peptide. Cancer Chemotherapy and Pharmacology, 2010. PMID 20182728
- 3.Davitt K, et al. The anti-cancer peptide PNC-27 induces necrosis in K562 leukemia cells. Annals of Clinical and Laboratory Science, 2014. PMID 25117093
Other weight classes: PNC-27 for a small / toy breed, PNC-27 for a medium breed.
Educational information — not veterinary medical advice
Everything on this page is compiled from published peer-reviewed literature for educational and research purposes. It does not establish a veterinarian-client-patient relationship (VCPR) and is not a substitute for veterinary examination and diagnosis. Most compounds described here are investigational or used off-label and are not FDA-approved for these purposes in dogs. Extralabel drug use in animals requires a valid VCPR and the direct supervision of a licensed veterinarian under AMDUCA (21 U.S.C. §360b). Dosing figures are reported as they appear in the source literature and are not recommendations.